Collaborators
ToxStrategies, LLC (US)
Timeline
Completion 3Q 2024
Additional information
Professional Presentations
Presented at Society of Toxicology Annual meeting, March 2024 in Salt Lake, UT.
Published Papers
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ToxStrategies, LLC (US)
Completion 3Q 2024
Presented at Society of Toxicology Annual meeting, March 2024 in Salt Lake, UT.
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Kick-off date: October 13, 2023; projected completion date: October 2024. A presentation and a paper describing study results are planned.
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Donated materials were received in December 2022. Two LDPE powders will be ready to market in 2024.
Two polyethylene microplastic materials were well-characterized to provide researchers with standardized materials for microplastic research. Analysis of materials included use of various polymer identification methods, including attenuated total reflectance-Fourier transform infrared spectroscopy (ATR FTIR), differential scanning calorimetry (DSC), uFTIR in reflectance mode, uRaman, py-GCMS, and HT-SEC.
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Funding and polymers were received in June 2020; Polymer Kit 1.0 was completed and launched onto the market in November 2020.
https://www.hpu.edu/cncs/cmdr/img/polymerkit1.0_marketingbrochure.pdf
Polymer Kit 1.0 – To Harmonize Plastic Pollution Research, Pacific Northwest Consortium on Plastics (virtual)
Polymer Kit 1.0 – Usefulness of Polymer Reference Materials in Marine Debris Analyses, SETAC North America (virtual)
Usefulness of Polymer Reference Materials in Marine Debris Analyses, PacifChem (virtual)
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Estimated publication date of study findings, March 2023.
A Novel Method for the Extraction of Microplastics from Marine Sediment, PacifiChem (virtual)
Effective microplastic extraction from deep sea sediment with an affordable and easily accessible density separation device, 7th International Marine Debris Conference (IMDC; Busan, Republic of Korea), September 18-23, 2022
Separation of microplastics from deep-sea sediment using an affordable, simple to use, and easily accessible density separation device, in review
Q3 2024
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Project is expected to be completed by September 2024 with publication of findings to follow in late 2024 or early 2025.
Microplastics are found in natural waters and in foods and drinks derived from these waters; microplastics are thus ingested and inhaled by humans and found in human tissues. Research on microplastics is challenging due to the labor-intensive protocols associated with isolating them from the environment and biological tissues, particularly at the size scales most relevant to human health (<20μm). Due to lack of well characterized commercial reference MPs, toxicity researchers often resort to commercially available polystyrene spheres, which are a poor model for the environmental microplastics that are most relevant to human health. As novel time-saving protocols, silicon nanomembrane analyses pipelines (SNAPs) are being used with reference and environmental microplastics. In these methods, raw samples are added to nanomembranes housed in filtration devices. The samples are serially processed here until ready for imaging or spectroscopic analysis. The SNAP protocols are reproducible and rigorous and will be used in future studies to characterize environmental microplastics.
The grant title for this project is, ‘Characterization of Highly Referenced Commercial Monodispersed Microplastics Test Materials.’
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Q2 2022 to Q3 2024 (milling, labeling and characterization),
Q3 to Q4 2024 (baseline testing)
Ingestion and inhalation are hypothesized to be the main routes of exposure of humans to microplastics. This project focuses on ingestion. Microplastics will be prepared by the micronization of commercial-grade forms of examples of each of the seven polymer types (polyethylene, polypropylene, polystyrene, (rigid)polyvinyl chloride, polycarbonate, polyamide-6 and polyethylene terephthalate – the latter in collaboration with PETCORE Europe). Some of these microplastics will be labelled by radioisotope1 labels – specifically, carbon-14 – to better track them during exposure experiments. The microplastics will be characterized using a variety of analytical techniques.
Further details are available here.
1https://www.sciencedirect.com/science/article/abs/pii/S0304389423020691
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